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Vitamin D3 (VD3) ELISA Kit

Hersteller Biomatik
Kategorie
Typ Elisa-Kit
Specific against General
Format 96 well plate
Applikationen ELISA
Menge 96T
ArtNr BM-EKU08284
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Detection Range
4.94-400 ng/mL
Sensitivity
1.85 ng/mL
Sample Type
Serum, plasma and other biological fluids
Assay Time
2 hours
Gene Name
Vitamin D3
Gene Aliases
Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
Assay Type
Competitive
Detection Method
Colorimetric
Shipping Condition
Blue ice
Storage Instructions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Vitamin D3 (VD3). No significant cross-reactivity or interference between Vitamin D3 (VD3) and analogues was observed.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin D3 (VD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin D3 (VD3) and unlabeled Vitamin D3 (VD3) (Standards or samples) with the pre-coated antibody specific to Vitamin D3 (VD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample.
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D3 (VD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D3 (VD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Metabolic pathway; Reproductive science; Nutrition metabolism; Bone metabolism;

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Menge: 96T
Lieferbar: In stock
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