Human Kallikrein 10 (KLK10) ELISA Kit (CLIA)

41.3 pg/mL

2 hours

5655

NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

Chemiluminescent

-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

8 months

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Kallikrein 10 (KLK10). A competitive inhibition reaction is launched between biotin labeled Kallikrein 10 (KLK10) and unlabeled Kallikrein 10 (KLK10) (Standards or samples) with the pre-coated antibody specific to Kallikrein 10 (KLK10). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kallikrein 10 (KLK10) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Kallikrein 10 (KLK10) level in the sample or standard.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.