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Human Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit (CLIA)

Hersteller Biomatik
Kategorie
Typ Clia
Specific against Human
Format 96 well plate
Applikationen CLIA
Menge 96T
ArtNr BM-EKU08846
Targets TNF
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 27150207
eClass 9.0 27150207
Lieferbar
Detection Range
468.75-30000 pg/mL
Sensitivity
15.5 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time
2 hours, 40min
UniProt ID
Q6IBA9
Gene ID
8743
Gene Name
Tumor Necrosis Factor Related Apoptosis Inducing Ligand
Gene Aliases
CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand
Assay Type
Sandwich
Detection Method
Chemiluminescent
Shipping Condition
Blue ice
Storage Instructions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) level in the sample or standard.;
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Apoptosis; Tumor immunity;

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Menge: 96T
Lieferbar: In stock
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