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Human Myeloid Progenitor Inhibitory Factor 2 (MPIF2) ELISA Kit (CLIA)

Hersteller Biomatik
Kategorie
Typ Clia
Specific against Human
Format 96 well plate
Applikationen CLIA
Menge 96T
ArtNr BM-EKU08909
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 27150207
eClass 9.0 27150207
Lieferbar
Detection Range
39.06-10000 pg/mL
Sensitivity
5.1 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time
2 hours, 40min
UniProt ID
O00175
Gene ID
6369
Gene Name
Myeloid Progenitor Inhibitory Factor 2
Gene Aliases
CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24
Assay Type
Sandwich
Detection Method
Chemiluminescent
Shipping Condition
Blue ice
Storage Instructions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2). No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloid Progenitor Inhibitory Factor 2 (MPIF2) level in the sample or standard.;
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Cytokine; Infection immunity;

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Menge: 96T
Lieferbar: In stock
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