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Human Deleted In Liver Cancer 1 (DLC1) ELISA Kit

ArtNr BM-EKU09233
Hersteller Biomatik
Menge 96 T
Kategorie
Typ Elisa-Kit
Format 96 well plate
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity 0.063 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias HP; ARHGAP7; STARD12; p122-RhoGAP; StAR-Related Lipid Transfer(START)Domain Containing 12; Rho GTPase-activating protein 7
Lieferbar
Shipping Temperature
Blue ice
Storage Conditions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Manufacturer - Research Area
Tumor immunity;
Manufacturer - Format
96 well plate
Restrictions
For research use only. Not for diagnostic procedures.
Precaution of use
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiration
8 months
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Test Principles
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deleted In Liver Cancer 1 (DLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deleted In Liver Cancer 1 (DLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deleted In Liver Cancer 1 (DLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deleted In Liver Cancer 1 (DLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Assay Time
3 hours
Assay Type
Sandwich
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deleted In Liver Cancer 1 (DLC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deleted In Liver Cancer 1 (DLC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Detection Method
Colorimetric
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Manufacturer - Gene Name
Deleted In Liver Cancer 1
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Deleted In Liver Cancer 1 (DLC1). No significant cross-reactivity or interference between Deleted In Liver Cancer 1 (DLC1) and analogues was observed.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 T
Lieferbar: In stock
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