Human SPARC Like Protein 1 (SPARCL1) ELISA Kit

SPARC Like Protein 1

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

0.133 ng/mL

Colorimetric

Double-antibody Sandwich

Short term: 4CC; Long term: see manual.

8 months

This assay has high sensitivity and excellent specificity for detection of SPARC Like Protein 1 (SPARCL1).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPARC Like Protein 1 (SPARCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SPARC Like Protein 1 (SPARCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPARC Like Protein 1 (SPARCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPARC Like Protein 1 (SPARCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.