Mini Samples Mouse Interleukin 1 Theta (IL1q) ELISA Kit

Ice packs

96-Well Plate

The Stop Solution is acidic. Do not allow to contact skin or eyes.

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 1 Theta (IL1q). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 1 Theta (IL1q). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 1 Theta (IL1q), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 1 Theta (IL1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Double-antibody Sandwich

1. Prepare all reagents, samples and standards; 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37CC; 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37CC; 4. Aspirate and wash 3 times; 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 6. Aspirate and wash 5 times; 7. Add 90 uL Substrate Solution. Incubate 10-20 minutes at 37CC; 8. Add 50 uL Stop Solution. Read at 450nm immediately.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 1 Theta (IL1q).