Mouse Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit (CLIA)

Heat Shock 70kDa Protein 5

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

0.055 ng/mL

Chemiluminescent

Double-antibody Sandwich

Short term: 4CC; Long term: see manual.

8 months

This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5).

The microplate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heat Shock 70kDa Protein 5 (HSPA5) level in the sample or standard.;

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.