Hersteller |
Biomatik
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Menge |
50ug |
ArtNr |
BM-RPC20686-50ug |
Targets |
CDK7;;RIPK1 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Gene Name |
RIPK1 |
Alternative Names |
Cell death protein RIP Receptor-interacting protein 1 Short name: RIP-1 Serine/threonine-protein kinase RIP RIP, RIP1 |
Uniprot |
Q13546 |
Source |
Baculovirus |
Expression Region |
1-375aa |
AA Sequence |
MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCF HRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRL RHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLK AEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLK PENILVDNDFHIKIADLGLASFKMWSKLNNEEHNE LREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSD VYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGN RPDVDDITEYCPREIISLMKLCWEANPEARPTFPG IEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVV KRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLG MGPVEESWFAPSLEHPQEENEPSLQ |
Sequence Info |
Partial |
Tag Info |
N-terminal 10xHis-tagged and C-terminal Myc-tagged |
Theoretical MW |
46.4 kDa |
Purity |
>85% as determined by SDS-PAGE. |
Storage Buffer |
Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Endotoxin Level |
Not tested |
Biological Activity |
Not tested |
Shipping Condition |
Ice packs |
Storage |
Short term: -20CC; Long term: -80CC. Minimize freeze and thaw cycles. |
Expiry Date |
1 year |
Restriction |
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals. |
Relevance |
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. |
Function |
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. |
Subcellular location |
Cytoplasm, Cell membrane |
Protein Families |
Protein kinase superfamily, TKL Ser/Thr protein kinase family |
Paythway |
NF-kappaBsignalingpathway |
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