Recombinant Human Protein-arginine deiminase type-4 (PADI4)

HL-60 PAD Peptidylarginine deiminase IV Protein-arginine deiminase type IV PAD4, PADI5, PDI5

E.coli

MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPE DCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPL DPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVK ALLYLTGVEISLCADITRTGKVKPTRAVKDQRTWT WGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSE DLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDK VRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKH NMDFYVEALAFPDTDFPGLITLTISLLDTSNLELP EAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFE NEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQD EMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVM GPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG KEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQ VQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLL LASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQK IKNILSNKTLREHNSFVERCIDWNRELLKRELGLA ESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKH LGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFI NDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP

N-terminal 10xHis-tagged and C-terminal Myc-tagged

>85% as determined by SDS-PAGE.

Not tested

Ice packs

1 year

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

Rheumatoid arthritis (RA)

Protein arginine deiminase family