Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active)

SHH, , , Sonic hedgehog protein C-product Sonic hedgehog protein 27 kDa product, ],

E.coli

GPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTL GASGR YEGKITRNSE RFKELTPNYN PDIIFKD EEN TGADRLMTQR CKDKLNALAI SVMNQWPGV K LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HC SVKAENSV AAKSGG

Tag-Free

>95% as determined by SDS-PAGE and HPLC.

Less than 1.0 EU/ug as determined by LAL method.

Ice packs

1 year

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269|PubMed:14687547, ECO:0000269|PubMed:7736596}.

Sonic hedgehog protein N-product: Cell membrane, Lipid-anchor

Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina.