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Rabbit antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor): affinity purified

Rabbit antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor): affinity purified

ArtNr	R-1675-100
Hersteller	Biosensis
Menge	100ug
Kategorie	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody
Applikationen	WB, IF, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ARMET, ARP
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Sub category	Neurotrophic Factors
products_extra_fields_value	MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinsona€s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
products_quantity	200
storage	Store antibody stock solution at +4AC upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
purityisotype	Affinity purified.
formulation	Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
immunogen	Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
crossreactivity	No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
conality	Rabbit
specificity	MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
reconstitution	Store antibody stock solution at +4AC upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally. Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.

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