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Chicken polyclonal antibody to Neurofilament Heavy phosphorylated

Chicken polyclonal antibody to Neurofilament Heavy phosphorylated

ArtNr	C-1386-50
Hersteller	Biosensis
Menge	50ul
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Western Blot ELISA Immunocytochemistry Primary Antibodies Neurodegeneration Markers Glial Cell Markers
Typ	Antibody Polyclonal
Applikationen	WB, ICC, ELISA
Specific against	other
Host	Chicken
Isotype	IgY
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	NF-H, NEFH, NF-200, NF200, N52, Neurofilament heavy polypeptide, Neurofilament triplet H protein, 200 kDa neurofilament protein, KIAA0845, NFH
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Sub category	Development-Cell Markers
products_extra_fields_value	Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody binds primarily to the phosphorylated axonal forms of NF-H.
products_quantity	200
storage	After reconstitution of lyophilised antibody, aliquot and store at -20AC for a higher stability. Avoid freeze-thaw cycles.
formulation	Lyophilised with 5% trehalose
immunogen	Purified bovine Neurofilament Heavy (NF-H)
crossreactivity	Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
conality	Chicken
specificity	This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H.
reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
specificreferences	1. Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22, 28(43):11003-14. 2. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul, 55(9):976-1000. 3. Shaw G. et al (2005) Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4, 336(4):1268-77.

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