MOAB-2 Mouse monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified

The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (Aβ, ) associated with the pathology characteristic of Alzheimera€s disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ, accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for Aβ, have been shown to actually detect intraneuronal APP and not Aβ, exclusively.

MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ, residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide.

MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice).

Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ, 40 and Aβ, 42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10.

In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, , distinct from Aβ, associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.

After reconstitution keep aliquots at -20AC to -70C for a higher stability. At 4AC keep up to one week, insulated, protected from light, use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.

Lyophilized, from a Protein A purified preparation in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% sodium azide, pH 7.2, contains 0.01% sodium azide as a preservative.

Human, Rat, other species not yet tested.By Dot blot, MOAB-2 detected rat Aβ, 40 and human Aβ, 40, albeit with less affinity than for Aβ, 42. {Youmans. KL et al 2012}

MOAB-2 detects preparations enriched in U-, O-, F-Aβ, 42, and U-Aβ, 40 by dot-blot, and is thus a pan-specific Aβ, antibody. However, MOAB-2 is selective for the more neurotoxic Aβ, 42 compared to Aβ, 40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects Aβ, 40 at 2.5 pmol but U-, O- and FAβ, b42 at antigen concentrations as low as ca. 0.1 pmol {Youmans. KL et al 2012}. MOAB-2 does not detect APP (Amyloid precursor protein).

1. K.L. Youmans et al (2012) Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody Mol Neurodegener. 2012 Mar 16, 7(1):8.
2. Tai LM et al (2013) Levels of soluble apolipoprotein E/amyloid-β, (Aβ, ) complex are reduced and oligomeric Aβ, increased with APOE4 and Alzheimer disease in a transgenic mouse model and human samples. J Biol Chem. 2013 Feb 22, 288(8):5914-26.