Rabbit antibody to phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha (&#945,-CaMKII, T253): whole serum

Calcium/calmodulin-stimulated protein kinase II (CaMKII) is composed of four different chains (alpha, beta, gamma, and delta) and is abundantly expressed in neurons. CaMKII is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity and cellular transport. The enzymatic function of CaMKII is regulated by its multiple phosphorylation sites and targeting to sub-cellular locations through interactions with protein binding partners. Phosphorylation of Thr253 has been identified in vivo and found to alter the interaction of CaMKII with binding partners, but not change its enzymatic activity. Thus, phosphorylation of Thr253 is suggested to modulate functional responses based on its binding partner and subsequently its sub-cellular localization.

After reconstitution of lyophilised antibody, aliquot and store at -20AC for a higher stability. Avoid freeze-thaw cycles.

Lyophilized

Not tested. Predicted from gene analysis to react with human and mouse α, -CaMKII.

Rat

1. Skelding KA et al (2012) J J Cereb Blood Flow Metab 32 (12), 2181-2192.
2. Skelding KA et al (2010) Cell Signal 22 (5), 759-769.
3. Gurd JW et al (2008) Brain Res 1218, 158-165.
4. Migues PV et al (2006) J Neurochem 98 (1), 289-299.