Rat NGF/NGF beta ELISA Kit (2 plates)

The rat NGF Kit is a sandwich ELISA. The capture antibody is a monoclonal rat NGF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known NGF concentration are added to these wells and allowed to complex with the bound NGF antibody. A biotinylated rat NGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat NGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Store at 4AC

The monoclonal used in this ELISA reacts with rat and mouse NGF but does not detect human NGF, thus this kit does show cross reactivity with mouse and potentially other rodent NGF proteins but shows less than 0.1% cross reactivity with human NGF