Rat NT3 ELISA Kit (2 plates)

The rat NT3 Kit is a sandwich ELISA. The capture antibody is a polyclonal NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat NT3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Store at 4AC

Human, Mouse, and Rat. Less than 2.5% reactivity with NT-4 and no detectable cross-reactivity with other cytokines.