BioTeZ Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein

The BioTeZ Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product.
The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22, 4 Å to increase the distance between the target molecule and the biotin groups. In the kit are 5 portions of Sulfo-NHS-LC Biotin reagent. One portion is used for a antibody amount of 1-10 mg or a protein amount of 1-5 mg.

all immuno assays

Streptavidin

12 month

If applicable mentioned in the product data sheet

37C, Biotin reagent at 2-8C

Biotinylation with Sulfo-NHS-LC of antibody / protein, coupling reaction with target molecules through the reaction with primary amino groups

Pipettes or pipetting equipment, vials for dilution, distilled or deionised water