10-Acetyl-3, 7-dihydroxyphenoxazine

Fluorescence Microscopy|Super-Resolution Imaging|Enzyme Substrates>Other Enzyme Substrates

10-Acetyl-3, 7-dihydroxyphenoxazine (N-Acetyl-3, 7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H₂O₂(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H₂O₂ is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.

• Detect HRP in immunoassays
• Detect trace amounts of hydrogen peroxide (H₂O₂)
• Detect using fluorometer or microplate reader
• λ _Ex/λ _Em(pH 9)= 571/585 nm (end product)
• ε = 54, 000 (end product)
• White to off-white solid soluble in DMSO or DMF
• Store desiccated at -20° C and protect from light
• C₁₄H₁₁CNO₄
• MW: 257.24
• [119171-73-2]

Amplex Red is a registered trademark of Thermo Fisher Scientific.

Store with desiccant; Protect from light

Stable for at least 1 month

Chemistry reagents or solutions