ArtNr |
B-23007 |
Hersteller |
Biotium
|
Menge |
1 ML |
Kategorie |
|
Typ |
Cells, Tissue Culture |
Specific against |
other |
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41105330 |
Gefahren-Info |
Transport hazard class for SDS: 3 |
Lieferbar |
|
Manufacturer - Category |
Buffers, Accessories, &; Background Reducers>Blocking Buffers &; Background Reducers|Fluorescence Microscopy |
Storage Conditions |
Room Temperature |
Description |
Features:
- Eliminates lipofuscin autofluorescence
- Reduces autofluorescence from non-lipofuscin sources
- Doesn't cause high background, unlike Sudan Black B
- Can be used before or after immunofluorescence staining
- Clears the way for fluorescence imaging of human and aged animal tissues
TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy, and accumulate in a variety of cell and tissue types with age. Consequently, imaging of specific immunofluorescence signal in certain adult human tissues or aged animal tissues can be virtually impossible unless methods are employed to quench or mask lipofuscin fluorescence.
Traditionally, Sudan Black B has been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However, while it masks the autofluorescence from lipofuscin, Sudan Black B also introduces uniform non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths. Now Biotium has developed TrueBlack® as a superior alternative to Sudan Black B. TrueBlack® quenches lipofuscin fluorescence with far less increase in red/far-red background fluorescence. TrueBlack® treatment can be performed before or after immunostaining. It is rapid, simple, and has minimal effect on signal from fluorescent antibodies or nuclear counterstains, thus preserving the signal-to-noise ratio of the immunostaining.
TrueBlack® effectively eliminates lipofuscin autofluorescence in tissues like human and mouse brain and retina. TrueBlack® also can reduce autofluorescence from other sources, such as collagen, elastin, red blood cells, and general background fluorescence. It is not as effective at quenching these sources of autofluorescence as it is for lipofuscin, but it can improve background in human and non-human tissue types. TrueBlack® also has been used to quench background fluorescence on polycarbonate filters used as cell supports for imaging cytometry. |
Product origin |
Synthetic |
Undated stability guarantee in PI |
12 months |
Stability at RT during shipping (protected from light) |
Stable at RT |
UN Number for SDS and label |
UN2265 |
Transport hazard class for SDS |
3 |
Packing group for SDS |
III |
Hazard pictogram (for SDS and label) |
GHS07 exclamation mark, GHS08 systemic health hazards |
Signal word (for SDS and label) |
Danger |
UNSPSC Commodity |
41105329 |
UNSPSC Commodity Title |
Blocking agents |
Classified or regulated chemicals |
Dimethyl formamide, CAS 68-12-2, 98% |
Hazard statements for SDS |
H312 Harmful in contact with skin H319 causes serious eye irritation H332 harmful if inhaled H360D may damage the unborn child |
Prop 65 Chemical - Reproductive Risk (if applicable) |
Dimethyl formamide, CAS 68-12-2 |
Requires Prop 65 Labeling (Y or blank) |
Yes |
Prop 65 Chemical - Cancer Risk (if applicable) |
Dimethyl formamide, CAS 68-12-2 |
Classified or regulated chemicals |
Dimethyl formamide, CAS 68-12-2, 98% |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.