DiO/DPA Membrane Potential Detection Kit

Cell & Organelle Stains>Membrane Potential & Nerve Terminal Dyes

The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET), where DiO is a "stationary" FRET donor while DPA acts as a potentiometric FRET acceptor. The DiO/DPA system has been reported to produce a fluorescence signal change of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change. The DiO/DPA membrane potential detection kit includes a vial of the green fluorescent membrane dye DiOC16(3) in DMSO (1 mL at 2 mM) and a vial of the anionic membrane quencher dye DPA (dipicrylamine) in DMSO (1 mL at 20 mM).

Protect from light;

Stable for at least 1 month

1.1D

41116111

Dimethylsulfoxide, CAS 67-68-5, >99% (kit components A and B); Dipicrylamine, CAS 2844-92-0, >1% (kit component B)

Dimethylsulfoxide, CAS 67-68-5, >99% (kit components A and B); Dipicrylamine, CAS 2844-92-0, >1% (kit component B)