DAPI, 10mg/mL in H2O

Fluorescence Microscopy|Nuclear Counterstains|Super-Resolution Imaging|Cell & Organelle Stains>Nuclear Stains &; DNA/RNA Dyes|Microbiology>Other Bacterial Stains|PCR &; DNA Amplification>DNA Binding Dyes

DAPI (4', 6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H₂O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye. 

 
• Classic blue nuclear counterstain for fixed cells
 
• Can be used at higher concentrations to stain live cells
 
• &lamba; _Ex/&lamba; _Em (with DNA) = 358/461 nm
 
• Yellow solution
 
• Store at 4°C and protect from light
 
• C₂₂H₂₇N₅O₆
 
• MW: 457.49
 

Protect from light;

Stable for at least 1 month

Cytology reagents or solutions or stains

DAPI dilactate, CAS 28718-91-4, 1%