DPA

Cell & Organelle Stains>Membrane Potential &; Nerve Terminal Dyes

Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes. When applied to cells, DPA localizes at the interface of cytoplasmic membrane and aqueous phase in a manner sensitive to membrane potential change. A recent application of DPA is to measure cell membrane potential change by combining with the fluorescent donor dye DiOC16. The DiO/DPA system has been reported to produce a fluorescence signal change of > 56% in HEK-293 cells and > 25% in neuronal cultures and brain slices per 100 mV membrane potential change. Please also see the DiO/DPA Membrane Potential Detection Kit.    Yellow solid soluble in DMSO at > 30mg/mL 

 
• &lamba; abs (MeOH) = 406 nm
 
• Store at 4°C
 
• MWt: 439.21
 
• [131-73-7]
 

   

Store with desiccant; Protect from light

Stable for at least 1 month

1.1D

Danger

Cytology reagents or solutions or stains

Expl. 1.1; H201, Explosive; mass explosion hazard.
Acute Tox. 2; H300, Fatal if swallowed.
Acute Tox. 1; H310, Fatal in contact with skin.
Acute Tox. 2; H330, Fatal if inhaled.
STOT RE 2; H373, May cause damage to organs through prolonged or repeated exposure.
Aquatic Chronic 2; H411, Toxic to aquatic life with long lasting effects.