Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®488A Tyramide

Blue ice

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure.    CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Green fluorescent CF® 488A has excitation/emission at 490/515 nm.    Kit Components: 

 
• CF® 488A tyramide stock solution
 
• HRP Goat anti-mouse IgG
 
• BSA for blocking
 
• Amplification buffer
 
• Hydrogen peroxide 30% stablized solution
 

    We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

IgG from domesticated goat serum (Capra aegagrus hircus); BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana)

6 months

2014

II

Danger

Histology test kits or supplies

H271 May cause fire or explosion, strong oxidizer
H302 Harmful if swallowed
H314 Causes severe burns and eye damage
H332 Harmful if inhaled

Hydrogen peroxide 30%, CAS 7722-84-1 (component 99823-100uL); Dimethylsulfoxide >99%, CAS 67-68-5 (tyramide component)