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MYL9/MYL12A (P13) polyclonal antibody

Hersteller Bioworld Technology, Inc
Kategorie
Typ Antibody Polyclonal
Specific against Human, Mouse, Rat
Applikationen WB, IF, IHC
Menge 100ul
Host Rabbit
ArtNr BS1552-100
Targets MYH14
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Swiss-Prot
P24844/P19105
Application all
WB: 1:500ca.1:1000
IHC: 1:50ca.1:200
IF: 1:50ca.1:200
Background
Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.
Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity
MYL9/MYL12A (P13) pAb detects endogenous levels of MYL9 and MYL12A protein.
WB
Western blot (WB) analysis of MYL9/MYL12A (P13) pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
IHC
Immunohistochemistry (IHC) analysis of MYL9/MYL12A (P13) pAb in paraffin-embedded human lung carcinoma tissue.
BiowMW
ca. 20 kDa
Note
For research use only, not for use in diagnostic procedure.
Alternative Name
Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, Short name=LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MLC2, MRLC1, MYRL2, Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12A, MLCB, MRLC3, RLC
Immunogen
Synthetic peptide, corresponding to the 10-40 of Human MYL9.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ul
Lieferbar: In stock
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