Anti-Transcriptional repressor CTCF CTCF Antibody

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Polyclonal

CTCF

recombinant protein

ProA affinity purified

Chromatin binding factor that binds to DNA sequence
specific sites. Involved in transcriptional regulation by binding
to chromatin insulators and preventing interaction between
promoter and nearby enhancers and silencers. Acts as
transcriptional repressor binding to promoters of vertebrate MYC
gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts
as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5
gene cluster and controls MHC class II gene expression. Plays an
essential role in oocyte and preimplantation embryo development by
activating or repressing transcription. Seems to act as tumor
suppressor. Plays a critical role in the epigenetic regulation.
Participates in the allele-specific gene expression at the
imprinted IGF2/H19 gene locus. On the maternal allele, binding
within the H19 imprinting control region (ICR) mediates maternally
inherited higher-order chromatin conformation to restrict enhancer
access to IGF2. Plays a critical role in gene silencing over
considerable distances in the genome. Preferentially interacts
with unmethylated DNA, preventing spreading of CpG methylation and
maintaining methylation-free zones. Inversely, binding to target
sites is prevented by CpG methylation. Plays a important role in
chromatin remodeling. Can dimerize when it is bound to different
DNA sequences, mediating long-range chromatin looping. Mediates
interchromosomal association between IGF2/H19 and WSB1/NF1 and may
direct distant DNA segments to a common transcription factory.
Causes local loss of histone acetylation and gain of histone
methylation in the beta-globin locus, without affecting
transcription. When bound to chromatin, it provides an anchor
point for nucleosomes positioning. Seems to be essential for
homologous X-chromosome pairing. May participate with Tsix in
establishing a regulatable epigenetic switch for X chromosome
inactivation. May play a role in preventing the propagation of
stable methylation at the escape genes from X- inactivation.
Involved in sister chromatid cohesion. Associates with both
centromeres and chromosomal arms during metaphase and required for
cohesin localization to CTCF sites. Regulates asynchronous
replication of IGF2/H19. Plays a role in the recruitment of CENPE
to the pericentromeric/centromeric regions of the chromosome
during mitosis (PubMed:26321640).

Boster Bio Anti-Transcriptional repressor CTCF CTCF Antibody catalog # A00132. Tested in WB, ICC/IF, IHC, IP applications. This antibody reacts with Human, Mouse, Rat, Zebrafish.

Ubiquitous. Absent in primary spermatocytes.