Anti-FMRP FMR1 Antibody

Primary Antibodies

71174 MW

WB: 1:5, 000-1:10, 000
ICC: 1:100-1:500
IHC: 1:50-1:200

Synaptic functional regulator FMR1

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Multifunctional polyribosome-associated RNA-binding
protein that plays a central role in neuronal development and
synaptic plasticity through the regulation of alternative mRNA
splicing, mRNA stability, mRNA dendritic transport and
postsynaptic local protein synthesis of a subset of mRNAs
(PubMed:16631377, PubMed:18653529, PubMed:19166269,
PubMed:23235829, PubMed:25464849). Plays a role in the alternative
splicing of its own mRNA (PubMed:18653529). Plays a role in mRNA
nuclear export (By similarity). Together with export factor NXF2,
is involved in the regulation of the NXF1 mRNA stability in
neurons (By similarity). Stabilizes the scaffolding postsynaptic
density protein DLG4/PSD-95 and the myelin basic protein (MBP)
mRNAs in hippocampal neurons and glial cells, respectively; this
stabilization is further increased in response to metabotropic
glutamate receptor (mGluR) stimulation (By similarity). Plays a
role in selective delivery of a subset of dendritic mRNAs to
synaptic sites in response to mGluR activation in a kinesin-
dependent manner (By similarity). Plays a role as a repressor of
mRNA translation during the transport of dendritic mRNAs to
postnyaptic dendritic spines (PubMed:11532944, PubMed:11157796,
PubMed:12594214, PubMed:23235829). Component of the CYFIP1-EIF4E-
FMR1 complex which blocks cap-dependent mRNA translation
initiation (By similarity). Represses mRNA translation by stalling
ribosomal translocation during elongation (By similarity). Reports
are contradictory with regards to its ability to mediate
translation inhibition of MBP mRNA in oligodendrocytes
(PubMed:23891804). Also involved in the recruitment of the RNA
helicase MOV10 to a subset of mRNAs and hence regulates microRNA
(miRNA)-mediated translational repression by AGO2
(PubMed:14703574, PubMed:17057366, PubMed:25464849). Facilitates
the assembly of miRNAs on specific target mRNAs (PubMed:17057366).
Plays also a role as an activator of mRNA translation of a subset
of dendritic mRNAs at synapses (PubMed:19097999, PubMed:19166269).
In response to mGluR stimulation, FMR1-target mRNAs are rapidly
derepressed, allowing for local translation at synapses (By
similarity). Binds to a large subset of dendritic mRNAs that
encode a myriad of proteins involved in pre- and postsynaptic
functions (PubMed:7692601, PubMed:11719189, PubMed:11157796,
PubMed:12594214, PubMed:17417632, PubMed:23235829,
PubMed:24448548). Binds to 5'-ACU[GU]-3' and/or 5'-[AU]GGA-3' RNA
consensus sequences within mRNA targets, mainly at coding sequence
(CDS) and 3'-untranslated region (UTR) and less frequently at 5'-
UTR (PubMed:23235829). Binds to intramolecular G-quadruplex
structures in the 5'- or 3'-UTRs of mRNA targets (PubMed:11719189,
PubMed:18579868, PubMed:25464849, PubMed:25692235). Binds to G-
quadruplex structures in the 3'-UTR of its own mRNA
(PubMed:7692601, PubMed:11532944, PubMed:12594214,
PubMed:15282548, PubMed:18653529). Binds also to RNA ligands
harboring a kissing complex (kc) structure; this binding may
mediate the association of FMR1 with polyribosomes
(PubMed:15805463). Binds mRNAs containing U-rich target sequences
(PubMed:12927206). Binds to a triple stem-loop RNA structure,
called Sod1 stem loop interacting with FMRP (SoSLIP), in the 5'-
UTR region of superoxide dismutase SOD1 mRNA (PubMed:19166269).
Binds to the dendritic, small non-coding brain cytoplasmic RNA 1
(BC1); which may increase the association of the CYFIP1-EIF4E-FMR1
complex to FMR1 target mRNAs at synapses (By similarity).
Associates with export factor NXF1 mRNA-containing
ribonucleoprotein particles (mRNPs) in a NXF2-dependent manner (By
similarity). Binds to a subset of miRNAs in the brain
(PubMed:14703574, PubMed:17057366). May associate with nascent
transcripts in a nuclear protein NXF1-dependent manner
(PubMed:18936162). In vitro, binds to RNA homopolymer;
preferentially on poly (G) and to a lesser extent on poly (U), but
not on poly (A) or poly (C) (PubMed:7688265, PubMed:7781595,
PubMed:12950170, PubMed:15381419, PubMed:8156595). Moreover, plays
a role in the modulation of the sodium-activated potassium channel
KCNT1 gating activity (PubMed:20512134). Negatively regulates the
voltage-dependent calcium channel current density in soma and
presynaptic terminals of dorsal root ganglion (DRG) neurons, and
hence regulates synaptic vesicle exocytosis (By similarity).
Modulates the voltage-dependent calcium channel CACNA1B expression
at the plasma membrane by targeting the channels for proteosomal
degradation (By similarity). Plays a role in regulation of MAP1B-
dependent microtubule dynamics during neuronal development (By
similarity). Recently, has been shown to play a translation-
independent role in the modulation of presynaptic action potential
(AP) duration and neurotransmitter release via large-conductance
calcium-activated potassium (BK) channels in hippocampal and
cortical excitatory neurons (PubMed:25561520). Finally, FMR1 may
be involved in the control of DNA damage response (DDR) mechanisms
through the regulation of ATR-dependent signaling pathways such as
histone H2AFX/H2A.x and BRCA1 phosphorylations (PubMed:24813610).

Boster Bio Anti-FMRP FMR1 Antibody catalog # A00192-1. Tested in WB, ICC/IF, IHC applications. This antibody reacts with Human, Mouse, Rat.