Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody

Primary Antibodies

33304 MW

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200

Cyclin-dependent-like kinase 5

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Proline-directed serine/threonine-protein kinase
essential for neuronal cell cycle arrest and differentiation and
may be involved in apoptotic cell death in neuronal diseases by
triggering abortive cell cycle re-entry. Interacts with D1 and D3-
type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin,
p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5,
SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5,
MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1,
huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several
neuronal development and physiological processes including
neuronal survival, migration and differentiation, axonal and
neurite growth, synaptogenesis, oligodendrocyte differentiation,
synaptic plasticity and neurotransmission, by phosphorylating key
proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2
(p39), especially in post-mitotic neurons, and promotes CDK5R1
(p35) expression in an autostimulation loop. Phosphorylates many
downstream substrates such as Rho and Ras family small GTPases
(e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins
(e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to
regulate neurite growth and/or spine morphogenesis. Phosphorylates
also exocytosis associated proteins such as MCAM/MUC18, SEPT5,
SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated
proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In
the mature central nervous system (CNS), regulates
neurotransmitter movements by phosphorylating substrates
associated with neurotransmitter release and synapse plasticity;
synaptic vesicle exocytosis, vesicles fusion with the presynaptic
membrane, and endocytosis. Promotes cell survival by activating
anti-apoptotic proteins BCL2 and STAT3, and negatively regulating
of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response
to genotoxic and oxidative stresses enhances its stabilization by
preventing ubiquitin ligase-mediated proteasomal degradation, and
induces transactivation of p53/TP53 target genes, thus regulating
apoptosis. Phosphorylation of p35/CDK5R1 enhances its
stabilization by preventing calpain-mediated proteolysis producing
p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal
degradation. During aberrant cell-cycle activity and DNA damage,
p25/CDK5 activity elicits cell-cycle activity and double-strand
DNA breaks that precedes neuronal death by deregulating HDAC1. DNA
damage triggered phosphorylation of huntingtin/HTT in nuclei of
neurons protects neurons against polyglutamine expansion as well
as DNA damage mediated toxicity. Phosphorylation of PXN reduces
its interaction with PTK2/FAK1 in matrix-cell focal adhesions
(MCFA) during oligodendrocytes (OLs) differentiation. Negative
regulator of Wnt/beta-catenin signaling pathway. Activator of the
GAIT (IFN-gamma-activated inhibitor of translation) pathway, which
suppresses expression of a post-transcriptional regulon of
proinflammatory genes in myeloid cells; phosphorylates the linker
domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-
dependent manner, the initial event in assembly of the GAIT
complex. Phosphorylation of SH3GLB1 is required for autophagy
induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in
response to osmotic stress mediates its rapid nuclear
localization. MEF2 is inactivated by phosphorylation in nucleus in
response to neurotoxin, thus leading to neuronal apoptosis. APEX1
AP-endodeoxyribonuclease is repressed by phosphorylation,
resulting in accumulation of DNA damage and contributing to
neuronal death. NOS3 phosphorylation down regulates NOS3-derived
nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-
dependent degradation and thus leads to cytoskeletal
reorganization. May regulate endothelial cell migration and
angiogenesis via the modulation of lamellipodia formation.
Involved in dendritic spine morphogenesis by mediating the EFNA1-
EPHA4 signaling. The complex p35/CDK5 participates in the
regulation of the circadian clock by modulating the function of
CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and
regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1
heterodimer in association with altered stability and subcellular
distribution.

Boster Bio Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody catalog # A00511-2. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Boster Bio Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody catalog # A00511-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.