Anti-Phospho-PKC delta (Y313) PRKCD Antibody

Primary Antibodies

77505 MW

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Protein kinase C delta type

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Calcium-independent, phospholipid- and diacylglycerol
(DAG)-dependent serine/threonine-protein kinase that plays
contrasting roles in cell death and cell survival by functioning
as a pro-apoptotic protein during DNA damage-induced apoptosis,
but acting as an anti-apoptotic protein during cytokine receptor-
initiated cell death, is involved in tumor suppression as well as
survival of several cancers, is required for oxygen radical
production by NADPH oxidase and acts as positive or negative
regulator in platelet functional responses. Negatively regulates B
cell proliferation and also has an important function in self-
antigen induced B cell tolerance induction. Upon DNA damage,
activates the promoter of the death-promoting transcription factor
BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription
and apoptosis. In response to oxidative stress, interact with and
activate CHUK/IKKA in the nucleus, causing the phosphorylation of
p53/TP53. In the case of ER stress or DNA damage-induced
apoptosis, can form a complex with the tyrosine-protein kinase
ABL1 which trigger apoptosis independently of p53/TP53. In cytosol
can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting
AKT1 and decreasing the level of X-linked inhibitor of apoptosis
protein (XIAP), whereas in nucleus induces apoptosis via the
activation of MAPK8 or MAPK9. Upon ionizing radiation treatment,
is required for the activation of the apoptosis regulators BAX and
BAK, which trigger the mitochondrial cell death pathway. Can
phosphorylate MCL1 and target it for degradation which is
sufficient to trigger for BAX activation and apoptosis. Is
required for the control of cell cycle progression both at G1/S
and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-
induced inhibition of cell cycle progression at G1/S phase by up-
regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin
CCNA2 promoter activity. In response to UV irradiation can
phosphorylate CDK1, which is important for the G2/M DNA damage
checkpoint activation. Can protect glioma cells from the apoptosis
induced by TNFSF10/TRAIL, probably by inducing increased
phosphorylation and subsequent activation of AKT1. Is highly
expressed in a number of cancer cells and promotes cell survival
and resistance against chemotherapeutic drugs by inducing cyclin
D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-
survival pathways, including NF-kappa-B, AKT1 and MAPK1/3
(ERK1/2). Can also act as tumor suppressor upon mitogenic
stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-
phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-
phox) phosphorylation and activation of NADPH oxidase activity,
and regulates TNF-elicited superoxide anion production in
neutrophils, by direct phosphorylation and activation of NCF1 or
indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also
play a role in the regulation of NADPH oxidase activity in
eosinophil after stimulation with IL5, leukotriene B4 or PMA. In
collagen-induced platelet aggregation, acts a negative regulator
of filopodia formation and actin polymerization by interacting
with and negatively regulating VASP phosphorylation. Downstream of
PAR1, PAR4 and CD36/GP4 receptors, regulates differentially
platelet dense granule secretion; acts as a positive regulator in
PAR-mediated granule secretion, whereas it negatively regulates
CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-
terminal and regulates the interaction between MUC1 and beta-
catenin. The catalytic subunit phosphorylates 14-3-3 proteins
(YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion (By
similarity). Phosphorylates ELAVL1 in response to angiotensin-2
treatment (PubMed:18285462).

Boster Bio Anti-Phospho-PKC delta (Y313) PRKCD Antibody catalog # A00822Y313. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.