Anti-AMPK Alpha 2 (Phospho-S345) PRKAA2 Antibody

Primary Antibodies

62320 MW

WB: 1:500-1:1, 000
ICC: 1:50-1:200
IHC: 1:50-1:100

5'-AMP-activated protein kinase catalytic subunit alpha-2

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Catalytic subunit of AMP-activated protein kinase
(AMPK), an energy sensor protein kinase that plays a key role in
regulating cellular energy metabolism. In response to reduction of
intracellular ATP levels, AMPK activates energy-producing pathways
and inhibits energy-consuming processes: inhibits protein,
carbohydrate and lipid biosynthesis, as well as cell growth and
proliferation. AMPK acts via direct phosphorylation of metabolic
enzymes, and by longer-term effects via phosphorylation of
transcription regulators. Also acts as a regulator of cellular
polarity by remodeling the actin cytoskeleton; probably by
indirectly activating myosin. Regulates lipid synthesis by
phosphorylating and inactivating lipid metabolic enzymes such as
ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and
cholesterol synthesis by phosphorylating acetyl-CoA carboxylase
(ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes,
respectively. Regulates insulin-signaling and glycolysis by
phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin
receptor/INSR internalization (PubMed:25687571). AMPK stimulates
glucose uptake in muscle by increasing the translocation of the
glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly
by mediating phosphorylation of TBC1D4/AS160. Regulates
transcription and chromatin structure by phosphorylating
transcription regulators involved in energy metabolism such as
CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP,
EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key
regulator of glucose homeostasis in liver by phosphorylating
CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the
cytoplasm. In response to stress, phosphorylates 'Ser-36' of
histone H2B (H2BS36ph), leading to promote transcription. Acts as
a key regulator of cell growth and proliferation by
phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient
limitation, negatively regulates the mTORC1 complex by
phosphorylating RPTOR component of the mTORC1 complex and by
phosphorylating and activating TSC2. In response to nutrient
limitation, promotes autophagy by phosphorylating and activating
ATG1/ULK1. In that process also activates WDR45 (PubMed:28561066).
AMPK also acts as a regulator of circadian rhythm by mediating
phosphorylation of CRY1, leading to destabilize it. May regulate
the Wnt signaling pathway by phosphorylating CTNNB1, leading to
stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and
SLC12A1. Plays an important role in the differential regulation of
pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or
ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4)
complexes, in response to glucose starvation. Can inhibit the non-
autophagy complex by phosphorylating PIK3C3 and can activate the
pro-autophagy complex by phosphorylating BECN1 (By similarity).

Boster Bio Anti-AMPK Alpha 2 (Phospho-S345) PRKAA2 Antibody catalog # A01420S345. Tested in WB, ICC/IF, IHC applications. This antibody reacts with Human, Mouse, Rat.