Anti-ATG3 Antibody

Primary Antibodies

35864 MW

ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

ATG3 autophagy related 3 homolog (S. cerevisiae)

ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3.

ATG3 Antibody is affinity chromatography purified via peptide column.

Autophagy

E2 conjugating enzyme required for the cytoplasm to
vacuole transport (Cvt), autophagy, and mitochondrial homeostasis.
Responsible for the E2-like covalent binding of
phosphatidylethanolamine to the C-terminal Gly of ATG8-like
proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-
ATG5 conjugate plays a role of an E3 and promotes the transfer of
ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE).
This step is required for the membrane association of ATG8-like
proteins. The formation of the ATG8-phosphatidylethanolamine
conjugates is essential for autophagy and for the cytoplasm to
vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also
acts as an autocatalytic E2-like enzyme, catalyzing the
conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing
a role in mitochondrial homeostasis but not in autophagy. ATG7
(E1-like enzyme) facilitates this reaction by forming an E1-E2
complex with ATG3. Promotes primary ciliogenesis by removing OFD1
from centriolar satellites via the autophagic pathway.

Boster Bio Anti-ATG3 Antibody (Catalog # A01768). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Bovine