ArtNr |
BOS-A02061-2-HRP |
Hersteller |
Boster
|
Menge |
100 ug |
Format |
Lyophilized |
Applikationen |
WB, FC, IF, ICC, ELISA |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
Isotype |
IgG |
Konjugat/Tag |
HRP |
Citations |
1. Avner, P., Bucan, M., Arnaud, D., Lehrach, H., Rapp, U. A-raf oncogene localizes on mouse X chromosome to region some 10-17 centimorgans proximal to hypoxanthine phosphoribosyltransferase gene. Somat. Cell Molec. Genet. 13: 267-272, 1987. 2. Beck, T. W., Huleihel, M., Gunnell, M., Bonner, T. I., Rapp, U. R. The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus. Nucleic Acids Res. 15: 595-609, 1987. |
Alias |
Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2 |
Lieferbar |
|
Manufacturer - Category |
Primary Antibodies |
Manufacturer - Isotype |
Rabbit IgG |
Storage Conditions |
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Observed Molecular Weight |
68 kDa |
Calculated Molecular weight |
37492 MW |
Clonality |
Polyclonal |
Application Details |
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml |
Manufacturer - Gene Name |
ARAF |
Gene Full Name |
A-Raf proto-oncogene, serine/threonine kinase |
Manufacturer - Specificity |
No cross reactivity with other proteins. |
Background |
Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. It is mapped to Xp11.3. This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. |
Immunogen |
E.coli-derived human ARAF recombinant protein (Position: E309-T413). |
Contents |
Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3. |
Purification |
Immunogen affinity purified. |
Reconstitution |
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml. |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Manufacturer - Research Category |
Signal Transduction |
Protein Name |
G2/mitotic-specific cyclin-B2 |
Protein Function |
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation. |
Subcellular Localization |
Cytosol. Mitochondrion. |
Description |
Boster Bio Anti-ARAF Antibody Picoband® catalog # A02061-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. |
Tissue Specificity |
Predominantly in urogenital tissues. |
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