Anti-Alpha-tubulin TUBA1A Antibody (biotin)

Primary Antibodies

50136 MW

Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Tubulin, alpha 1a

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and -Actin, this antibody makes an excellent loading control in immunoblots.

Biotin-Alpha-tubulin antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

1 mg/mL

Tubulin, alpha 1a

Cytoplasm, cytoskeleton.

Expressed at a high level in fetal brain.