Human Lipocalin-1 ELISA Kit PicoKine®

PicoKine® ELISA Kits

LCN1

Lipocalin-1 is a protein that in humans is encoded by the LCN1 gene. This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.

Human Lipocalin-1 PicoKine® ELISA Kit (96 Tests). Quantitate Human Lipocalin-1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 60 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1, 000 | 2, 000 | 4, 000 | 8, 000
O.D. | 0.088 | 0.133 | 0.199 | 0.307 | 0.484 | 0.829 | 1.254 | 1.911

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 239 | 213 | 234 | 221 | 227 | 17.28 | 7.6%
Sample 2 | 467 | 451 | 478 | 326 | 431 | 11.97 | 2.8%
Sample 3 | 3891 | 3425 | 3196 | 2685 | 3299 | 141.87 | 4.3%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.