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Human CD107a ELISA Kit PicoKine®

Human CD107a ELISA Kit PicoKine®

ArtNr	BOS-EK2230
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Neuroscience Molecular Targets for Neuroscience Protein Aggregation, Clearance & Misfolding Cell Biology Cell Status ELISA Autophagy Immunostaining
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<150 pg/ml
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	LAMP1
Manufacturer - Specificity	Natural and recombinant human LAMP1
Background	Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
Immunogen	Expression system for standard: NS0; Immunogen sequence: A29-M382
Description	Human CD107a PicoKine® ELISA Kit (96 Tests). Quantitate Human CD107a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1, 250 \| 2, 500 \| 5, 000 \| 10, 000 \| 20, 000 O.D. \| 0.005 \| 0.034 \| 0.068 \| 0.132 \| 0.339 \| 0.767 \| 1.213 \| 1.985
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 574 \| 899 \| 5292 \| 315 \| 1211 \| 5536 Standard deviation \| 19.17 \| 21.31 \| 346.1 \| 13.55 \| 54.98 \| 400.81 CV(%) \| 3.3% \| 2.4% \| 6.5% \| 4.3% \| 4.5% \| 7.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 574 \| 513 \| 455 \| 584 \| 532 \| 16.8 \| 3.2% Sample 2 \| 899 \| 902 \| 733 \| 982 \| 879 \| 47.64 \| 5.4% Sample 3 \| 5292 \| 8796 \| 6829 \| 7925 \| 7211 \| 494.64 \| 6.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	a polyclonal antibody from goat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-EK2230-datasheet.pdf

BOS-EK2230-safety-datasheet.pdf