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Human LPL ELISA Kit PicoKine®

Human LPL ELISA Kit PicoKine®

ArtNr	BOS-EK2238
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Metabolism Cholesterol & Lipid Metabolism ELISA Enzymes Fatty Acid Catabolism Immunostaining
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<40 pg/ml
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	LPL
Manufacturer - Specificity	Natural and recombinant human LPL
Background	LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.
Immunogen	Expression system for standard: CHO; Immunogen sequence: A28-G475
Description	Human LPL PicoKine® ELISA Kit (96 Tests). Quantitate Human LPL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 40 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 78 \| 156 \| 312 \| 625 \| 1, 250 \| 2, 500 \| 5, 000 O.D. \| 0.023 \| 0.159 \| 0.185 \| 0.326 \| 0.643 \| 1.072 \| 1.329 \| 1.872
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 137 \| 201 \| 2403 \| 127 \| 163 \| 2010 Standard deviation \| 9.55 \| 9.27 \| 160.76 \| 5.82 \| 11.8 \| 98.49 CV(%) \| 7% \| 4.6% \| 6.7% \| 4.6% \| 7.2% \| 4.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 137 \| 100 \| 85 \| 132 \| 114 \| 3.71 \| 3.3% Sample 2 \| 201 \| 296 \| 166 \| 236 \| 225 \| 4.72 \| 2.1% Sample 3 \| 2403 \| 2329 \| 2427 \| 2205 \| 2341 \| 163.17 \| 7% *number of samples for each test n=16.
Capture antibody and Detection antibody	a polyclonal antibody from goat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-EK2238-datasheet.pdf

BOS-EK2238-safety-datasheet.pdf