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Human IL22BP ELISA Kit PicoKine®

Human IL22BP ELISA Kit PicoKine®

ArtNr	BOS-EK2245
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Cell Biology Cell Culture Cytokines ELISA Immunostaining
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<25 pg/ml
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	IL22RA2
Manufacturer - Specificity	Natural and recombinant human IL22RA2
Background	This gene encodes a member of the class II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. The encoded protein may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen	Expression system for standard: CHO; Immunogen sequence: T22-P231
Description	Human IL22BP PicoKine® ELISA Kit (96 Tests). Quantitate Human IL22BP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 46.9 \| 93.8 \| 187.5 \| 375 \| 750 \| 1, 500 \| 3, 000 O.D. \| 0.013 \| 0.036 \| 0.106 \| 0.202 \| 0.429 \| 0.806 \| 1.320 \| 1.800
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 54 \| 151 \| 1130 \| 58 \| 130 \| 1247 Standard deviation \| 2.11 \| 6.67 \| 44.41 \| 1.35 \| 5.34 \| 39.65 CV(%) \| 3.9% \| 4.4% \| 3.9% \| 2.3% \| 4.1% \| 3.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 54 \| 65 \| 64 \| 81 \| 66 \| 1.6 \| 2.4% Sample 2 \| 151 \| 158 \| 136 \| 134 \| 145 \| 2.92 \| 2% Sample 3 \| 1130 \| 1499 \| 964 \| 795 \| 1097 \| 30.06 \| 2.7% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-EK2245-datasheet.pdf

BOS-EK2245-safety-datasheet.pdf