Anti-Hsc70/HSPA8 Antibody Picoband®

Primary Antibodies

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

70898 MW

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Heat shock cognate 71 kDa protein

HSPA8 (heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a  heat shock protein  that in humans is encoded by the  HSPA8  gene. The  HSPA8  gene contains 9 exons and spans 5 kb. The deduced  HSPA8  protein has 646 amino acids and a predicted molecular mass of 70, 899 Da. The HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.

Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.

Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction

Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19- CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP.

Boster Bio Anti-Hsc70/HSPA8 Antibody catalog # PA1816. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Belongs to the heat shock protein 70 family.