ArtNr |
BOS-PA1639 |
Hersteller |
Boster
|
Menge |
100 ug/vial |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Lyophilized |
Applikationen |
WB, IHC, ICC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Sensitivity |
>5000 cells |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
DNA repair protein XRCC1;X-ray repair cross-complementing protein 1;XRCC1; |
Similar products |
complementing defective, X ray repair, DNA repair protein XRCC1 antibody, RCC antibody, X ray repair complementing defective repair in chinese hamster antibody, X ray repair complementing defective repair in chinese hamster cells 1 antibody, X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody, X ray repair cross complementing protein 1 antibody, repair in Chinese hamster antibody, X-ray repair cross-complementing protein 1 antibody, XRCC 1 antibody, Xrcc1 antibody, XRCC1_HUMAN antibody |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Cancer, Cancer Metabolism, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nucleotide Metabolism, Pathways and Processes, Response To Hypoxia |
Short Description |
Boster Bio Anti-XRCC1 Antibody catalog # PA1639. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-XRCC1 Antibody catalog # PA1639. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain. |
Gene Name |
XRCC1 |
Application Details |
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Application Notes |
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Predicted Reactivity |
Hamster |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
DNA repair protein XRCC1 |
Molecular Weight |
69477 MW |
Protein Function |
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. |
Subcellular Localization |
Nucleus. Accumulates at sites of DNA damage. |
Protein Name |
DNA repair protein XRCC1 |
Sequence Similarities |
Contains 2 BRCT domains. |
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