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Anti-ARHGEF1 Antibody Picoband®

Anti-ARHGEF1 Antibody Picoband®

ArtNr	BOS-PB10045
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Oncology Neuroscience Neural Signaling Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Primary Antibodies By Organ Testicular & Prostate Cancer small GTPases Rho Subfamily
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, FC, IF, IHC, ICC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	>5000 cells
Citations	1. Aasheim HC, Pedeutour F, Smeland EB (May 1997). "Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues". Oncogene 14 (14): 1747–52. 2. Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G (Nov 1996). "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase". J Biol Chem 271 (41): 25452–8.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Rho guanine nucleotide exchange factor 1;115 kDa guanine nucleotide exchange factor;p115-RhoGEF;p115RhoGEF;Sub1.5;ARHGEF1;
Lieferbar
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	120 kDa
Calculated Molecular weight	102435 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Mouse, Rat, Human, By Heat Immunocytochemistry/Immunofluorescence, 2?g/ml, Human Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells, Human
Manufacturer - Gene Name	ARHGEF1
Gene Full Name	Rho guanine nucleotide exchange factor 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Rho guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the ARHGEF1 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of human ARHGEF1, different from the related mouse and rat sequences by one amino acid.
Contents	Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Signal Transduction, Signaling Pathway, G Protein Signaling, Regulators, Small G Proteins
Protein Name	Rho guanine nucleotide exchange factor 1
Protein Function	Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation.
Subcellular Localization	Cytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.
Description	Boster Bio Anti-ARHGEF1 Antibody Picoband® catalog # PB10045. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Ubiquitously expressed.

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BOS-PB10045-datasheet.pdf

BOS-PB10045-safety-datasheet.pdf