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Anti-GM-CSF/CSF2 Antibody Picoband™

Anti-GM-CSF/CSF2 Antibody Picoband™

Hersteller	Boster
Kategorie	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Specific against	Mouse
Isotype	IgG
Format	Lyophilized
Applikationen	WB, IHC
Menge	100 ug/vial
Host	Rabbit
ArtNr	BOS-PB9003
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category	Cytokines, Immunology, Innate Immunity
Short Description	Boster Bio Anti-GM-CSF/CSF2 Antibody Picoband™ catalog # PB9003. Tested in IHC, WB applications. This antibody reacts with Mouse.
Description	Boster Bio Anti-GM-CSF/CSF2 Antibody Picoband™ catalog # PB9003. Tested in IHC, WB applications. This antibody reacts with Mouse.
Background	GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.
Gene Name	CSF2
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat Western blot, 0.1-0.5μg/ml, Mouse
Application Notes	WB: The detection limit for GM-CSF is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Immunogen	E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Granulocyte-macrophage colony-stimulating factor
Molecular Weight	16091 MW
Protein Function	Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subcellular Localization	Secreted.
Protein Name	Granulocyte-macrophage colony-stimulating factor
Sequence Similarities	Belongs to the GM-CSF family.

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