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Anti-Cdc20 Antibody

Anti-Cdc20 Antibody

Hersteller	Boster
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Western Blot Primary Antibodies
Typ	Antibody Polyclonal
Specific against	Mouse, Rat
Isotype	IgG
Format	Lyophilized
Applikationen	WB
Menge	100 ug/vial
Host	Rabbit
ArtNr	BOS-PA2124
Targets	CDC20
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Background	The cell-division cycle protein 20, also known as p55CDC, is an essential regulator of cell division that is encoded by the CDC20 gene in humans. The chromosomal assignment of human CDC20 is 1p34.2. CDC20 is a component of the mammalian cell cycle mechanism. CDC20 appears to act as a regulatory protein interacting with many other proteins at multiple points in the cell cycle. This gene's most important function is to activate the anaphase promoting complex(APC), a large 11-13 subunit complex that initiates chromatid separation and entrance into anaphase.
Concentration	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Cross reactivity	No cross reactivity with other proteins
Description	Rabbit IgG polyclonal antibody for Cell division cycle protein 20 homolog(CDC20) detection. Tested with WB in Rat; Mouse.
Gene full name	Cell division cycle protein 20 homolog
Gene name	Cdc20
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cdc20(20-36aa IPNAPVARWQRKAKEAT), different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids.
MW	54816 MW
Predicted reactivity	Hamster
Protein function	Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
Protein name	Cell division cycle protein 20 homolog
Purification	Immunogen affinity purified.
Recommended detection ystems	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Research category	\|cell biology\|proteolysis / ubiquitin\|proteasome / ubiquitin\|apc/c
Sequence similarities	Belongs to the WD repeat CDC20/Fizzy family.
Storage	At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time.Avoid repeated freezing and thawing.
Subcellular localization	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .

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