|
Hersteller |
Boster
|
|
Kategorie |
|
|
Typ |
Antibody Polyclonal |
|
Specific against |
other |
|
Isotype |
IgG |
|
Format |
Lyophilized |
|
Applikationen |
WB, IHC |
|
Menge |
100ug/vial |
|
ArtNr |
BOS-A00081-3 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Description short |
Rabbit IgG polyclonal antibody for APP/C99 detection. Tested with WB, IHC-P in Human. |
| Specificity |
No cross reactivity with other proteins. |
| Application details |
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml |
| Notes |
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. |
| Immunogen |
A synthetic peptide corresponding to a sequence of human APP/C99(DAEFRHDSGYEVHHQKLVFFAEDVGSNK). |
| Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Purification |
Immunogen affinity purified. |
| Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Storage |
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing. |
| Relevant detection systems |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
| Background |
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. It is mapped to 21q21.3. This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Gene name |
APP |
| Protein name |
Amyloid-beta A4 protein |
| Gene full name |
amyloid beta precursor protein |
| Synonyms |
Amyloid-beta A4 protein; ABPP; APPI; APP; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II; S-APP-alpha; S-APP-beta; Beta-secretase C-terminal fragment; Beta-CTF; Abeta42; Beta-APP42; Abeta40; Beta-APP40; Alpha-secretase C-terminal fragment; Alpha-CTF; Amyloid intracellular domain 59; AICD-59; AID(59); Gamma-CTF(59); Amyloid intracellular domain 57; AICD-57; AID(57); Gamma-CTF(57); Amyloid intracellular domain 50; AICD-50; AID(50); Gamma-CTF(50); APP; A4; AD1 |
| Uniprot |
P05067 |
| Entrez gene id |
351 |
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