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Anti-Cox-2 Antibody Europäischer Partner

ArtNr BOS-A00084-1
Hersteller Boster
Menge 100 ul
Kategorie
Typ Antibody
Format Liquid
Applikationen WB
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Dry ice Yes
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias COX2, Prostaglandin G/H synthase 2, Cyclooxygenase-2, Prostaglandin-endoperoxide synthase 2, Prostaglandin H2 synthase 2, PGH synthase 2, PGHS-2
Lieferbar
Manufacturer - Category
Primary Antibodies
Manufacturer - Isotype
Antiserum
Storage Conditions
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Manufacturer - Format
Liquid (sterile filtered)
Clonality
Polyclonal
Application notes
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown.
Application Details
ELISA: 1:10, 000 - 1:50, 000
IHC: User optimized
WB: 1:5, 000 - 1:10, 000
Manufacturer - Gene Name
PTGS2
Background
COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases.  Cox-2 is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, Cox-2 is therefore of interest in studying immune response regulation.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human Cox-2.
Contents
0.01% (w/v) Sodium Azide
Purification
This antiserum is directed against human Cox-2. Two different isoforms of the enzyme are known, Cox-1 and Cox-2. A BLAST analysis was used to suggest cross-reactivity with Cox-2 in human, mouse and rat based on a homology with the immunizing sequence and should not cross react with Cox-1. Reactivity against homologues from other sources is not known. Reactivity of this antibody with Cox-2 from other species is unknown.

 
Concentration
55 mg/mL by Refractometry
Short Description
Boster Bio Anti-Cox-2 Antibody (Catalog # A00084-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Description
Boster Bio Anti-Cox-2 Antibody (Catalog # A00084-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
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