|
Hersteller |
Boster
|
|
Kategorie |
|
|
Typ |
Antibody Polyclonal |
|
Specific against |
other |
|
Isotype |
IgG |
|
Format |
Lyophilized |
|
Applikationen |
WB, FC, IHC, ICC |
|
Menge |
100ug/vial |
|
ArtNr |
BOS-A00142-1 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Description short |
Rabbit IgG polyclonal antibody for BCL6 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human; Mouse; Rat. |
| Specificity |
No cross reactivity with other proteins. |
| Application details |
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml
Immunohistochemistry(Frozen Section)|0.5-1μ g/ml
Immunocytochemistry|0.5-1μ g/ml
Flow Cytometry|1-3ug/1x106 cells |
| Notes |
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. |
| Immunogen |
A synthetic peptide corresponding to a sequence of human BCL6 (ASPADSCIQFTRHASDVLLNLNRLRSRDILTD). |
| Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Purification |
Immunogen affinity purified. |
| Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Storage |
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing. |
| Relevant detection systems |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC. |
| Background |
B-cell lymphoma 6 protein is a protein that in humans is encoded by the BCL6 gene. Like BCL2, BCL3, BCL5, BCL7A, BCL9 and BCL10, it has clinical significance in lymphoma. The protein encoded by this gene is an evolutionarily conserved zinc finger transcription factor and contains an N-terminal POZ/BTB domain. This protein acts as a sequence-specific repressor of transcription and has been shown to modulate the STAT-dependent Interleukin 4 (IL-4) responses of B cells. This protein can interact with several corepressor complexes to inhibit transcription. This gene is found to be frequently translocated and hypermutated in diffuse large B cell lymphoma (DLBCL) and contributes to the pathogenesis of DLBCL. An exon 7 skipping splice variant encodes a shorter form of the protein which lacks the first two zinc fingers of the DNA binding domain. |
| Gene name |
BCL6 |
| Protein name |
B-cell lymphoma 6 protein |
| Gene full name |
B-cell CLL/lymphoma 6 |
| Synonyms |
B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL6; BCL5; LAZ3; ZBTB27; ZNF51 |
| Uniprot |
P41182 |
| Entrez gene id |
604 |
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