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Anti-Cyclin B1 CCNB1 Antibody

Anti-Cyclin B1 CCNB1 Antibody

ArtNr	BOS-A00745
Hersteller	Boster
Menge	100 ul
Kategorie	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Blastoma Carcinoma Cell Biology Cell Status Immunohistochemistry Immunoprecipitation Western Blot Proliferation Primary Antibodies By Organ Bladder Cancer
Typ	Antibody
Format	Liquid
Applikationen	WB, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Dry ice	Yes
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	cyclin B1, G2/mitotic-specific cyclin-B1
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Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Antiserum
Storage Conditions	Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Observed Molecular Weight	48337 MW
Manufacturer - Format	Liquid (sterile filtered)
Clonality	Polyclonal
Application notes	Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity. Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user. Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.
Application Details	ELISA: 1:2, 000 - 1:10, 000 IHC: 1:500 IP: 1:100 WB: 1:500 - 1:1, 000
Manufacturer - Gene Name	CCNB1
Gene Full Name	G2/mitotic-specific cyclin-B1
Background	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Immunogen	Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Contents	0.01% (w/v) Sodium Azide
Purification	This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross-reactivity with Cyclin B1 from other species may also occur.
Concentration	80 mg/mL by Refractometry
Protein Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subcellular Localization	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Short Description	Boster Bio Anti-Cyclin B1 CCNB1 Antibody (Catalog # A00745). Tested in IHC, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Cyclin B1 CCNB1 Antibody (Catalog # A00745). Tested in IHC, WB applications. This antibody reacts with Human.

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BOS-A00745-datasheet.pdf

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