Anti-MAD2L1 Antibody

ArtNr	BOS-A00785
Hersteller	Boster
Menge	100 ug
Kategorie	Neuroscience Neural Lineage Markers Antibodies Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody
Format	Liquid
Applikationen	WB, ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Dry ice	Yes
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MAD2L1, MAD2, MAD2-like protein 1, HsMAD2, Mitotic arrest deficient 2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Manufacturer - Format	Liquid (sterile filtered)
Clonality	Polyclonal
Application notes	This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract.
Application Details	ELISA: 1:2, 000 - 1:10, 000 IF Microscopy: User optimized WB: 1:500 - 1:2, 000
Manufacturer - Gene Name	MAD2L1
Background	MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. This protein has a nuclear localization.
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal region near amino acid residues 1-25 of Human MAD2L1 protein.
Contents	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Purification	This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100% homology for the immunogen sequence. Cross-reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90% positive by BLAST). Cross-reactivity with MAD2L1 homologues from other sources has not been determined.
Concentration	1.19 mg/mL by UV absorbance at 280 nm
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Division, Nucleus, Spindle, Subcellular Markers, Tags & Cell Markers
Short Description	Boster Bio Anti-MAD2L1 Antibody (Catalog # A00785). Tested in ELISA, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-MAD2L1 Antibody (Catalog # A00785). Tested in ELISA, WB applications. This antibody reacts with Human.

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BOS-A00785-datasheet.pdf

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