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Anti-HDAC-5 (internal) Antibody Europäischer Partner

ArtNr BOS-A01230
Hersteller Boster
Menge 100 ug
Kategorie
Typ Antibody
Format Liquid
Applikationen WB, ELISA, Dot
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Rabbit
Isotype IgG
Dry ice Yes
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HDAC-5, HDAC5, HDAC 5, HD 5, histone deacetylase 5, Histone deacetylase-5, HD5, NY-CO-9
Lieferbar
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
Observed Molecular Weight
121978 MW
Manufacturer - Format
Liquid (sterile filtered)
Clonality
Polyclonal
Application notes
Anti-HDAC5 antibody has been tested in ELISA, dot blot, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.
Application Details
ELISA: 1:20, 000 - 1:60, 000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 0.2 µg/ml
Manufacturer - Gene Name
HDAC5
Gene Full Name
Histone deacetylase 5
Background
HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
Immunogen
HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
Purification
Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.
Concentration
1.12 mg/mL by UV absorbance at 280 nm
Manufacturer - Research Category
Acetylation, Cardiovascular, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Hypertrophy, Signaling Pathways, Stem Cells
Protein Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Subcellular Localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.
Short Description
Boster Bio Anti-HDAC-5 (internal) Antibody (Catalog # A01230). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Mouse.
Description
Boster Bio Anti-HDAC-5 (internal) Antibody (Catalog # A01230). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Mouse.
Tissue Specificity
Ubiquitous.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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