Anti-RPS3 Antibody

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, IHC
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A01542-2
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Reactivity
Reacts with: human, mouse, rat
Description short
Rabbit IgG polyclonal antibody for RPS3 detection. Tested with WB, IHC-P in Human; Mouse; Rat.
Specificity
No cross reactivity with other proteins.
Application details
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence of human RPS3 (RKFVADGIFKAELNEFLTRELAED).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Background
40S ribosomal protein S3 is a protein that in humans is encoded by the RPS3 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Reference
1. Kenmochi, N., Kawaguchi, T., Rozen, S., Davis, E., Goodman, N., Hudson, T. J., Tanaka, T., Page, D. C. A map of 75 human ribosomal protein genes. Genome Res. 8: 509-523, 1998.
2. Pogue-Geile, K., Geiser, J. R., Shu, M., Miller, C., Wool, I. G., Meisler, A. I., Pipas, J. M.Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Molec. Cell. Biol. 11: 3842-3849, 1991.
3. Polakiewicz, R. D., Munroe, D. J., Jani Sait, S. N., Tycowski, K. T., Nowak, N. J., Shows, T. B., Housman, D. E., Page, D. C. Mapping of ribosomal protein S3 and internally nested snoRNA U15A gene to human chromosome 11q13.3-q13.5. Genomics 25: 577-580, 1995.
Gene Name
RPS3
Protein Name
40S ribosomal protein S3
Gene Full Name
ribosomal protein S3
Uniprot ID
P23396
Entrez GeneID
6188
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
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