Antibodies

Anti-METTL3/METTL3 Antibody

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, ELISA
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A01758-1
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Reactivity
Reacts with: human, mouse, rat
Description short
Rabbit IgG polyclonal antibody for METTL3 detection. Tested with WB, ICC/IF, Direct ELISA in Human; Mouse; Rat.
Specificity
No cross reactivity with other proteins.
Application details
Western blot|0.1-0.5μ g/ml
Immunocytochemistry/Immunofluorescence|2μ g/ml
Direct ELISA|0.1-0.5μ g/ml
Notes
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E. coli-derived human METTL3 recombinant protein (Position: Q423-D571).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for and ICC.
Background
N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by theMETTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Reference
1. Alarcon, C. R., Lee, H., Goodarzi, H., Halberg, N., Tavazoie, S. F. N(6)-methyladenosine marks primary microRNAs for processing. Nature 519: 482-485, 2015.
2. Barbieri, I., Tzelepis, K., Pandolfini, L., Shi, J., Millan-Zambrano, G., Robson, S. C., Aspris, D., Migliori, V., Bannister, A. J., Han, N., De Braekeleer, E., Ponstingl, H., Hendrick, A., Vakoc, C. R., Vassiliou, G. S., Kouzarides, T.Promoter-bound METTL3 maintains myeloid leukaemia by m(6)A-dependent translation control. Nature 552: 126-131, 2017.
3. Bujnicki, J. M., Feder, M., Radlinska, M., Blumenthal, R. M. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA:m6A methyltransferase. J. Molec. Evol. 55: 431-444, 2002.
Gene Name
METTL3
Protein Name
N6-adenosine-methyltransferase catalytic subunit
Gene Full Name
methyltransferase like 3
Uniprot ID
Q86U44
Entrez GeneID
56339
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
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